Time-Resolved Fluorometric Detection of DNA Using a Tetradentate .BETA.-Diketonate Europium Chelate as a Label.
نویسندگان
چکیده
منابع مشابه
Immunofluorometry of choriogonadotropin by time-resolved fluorescence spectroscopy, with a new europium chelate as label.
We describe a new "sandwich"-type non-isotopic immunoassay for human choriogonadotropin (hCG) in serum. In the assay, hCG is captured by a beta-subunit-specific monoclonal antibody, which is immobilized in a white microtiter well. The sandwich is completed by adding a second biotinylated monoclonal antibody specific for the whole hCG molecule. The degree of binding of biotinylated antibody, whi...
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The luminescent Eu complex, DTBTA-Eu, is highly stable in terms of ligand-metal dissociation, and can be labelled to amino groups of proteins and other bio-molecules. Owing to the long lifetime of Eu luminescence, time-resolved measurement of DTBTA-Eu removes background fluorescence and enhances the sensitivity of the labelled materials. This property was applied to time-resolved luminescence m...
متن کاملPolyvinylamine-streptavidin complexes labeled with a europium chelator: a universal detection reagent for solid-phase time resolved fluorometric applications.
OBJECTIVES To describe the synthesis and characteristics of a new streptavidin-based universal detection reagent which is multiply labeled with the europium chelate of 4,7-bis(chlorosulfophenyl)-1, 10-phenanthroline-2,9-dicarboxylic-acid (BCPDA). METHODS AND RESULTS Polyvinylamine (PVA) was first labeled with biotin(b) and then with BCPDA to create (b)(x)-PVA-(BCPDA)(y). By mixing controlled ...
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A time resolved fluorometric immunoassay (TRFIA) has been developed and compared with an in house enzyme linked immunosorbent assay (ELISA) and commercial ELISA (Bindazyme) for the detection of tetanus antitoxin in human sera. A panel of 132 sera submitted for routine testing was used. Scatterplots showed a high degree of correlation between all three assays, although some divergence of results...
متن کاملA Novel Europium Chelate Coated Nanosphere for Time-Resolved Fluorescence Immunoassay
A novel europium ligand 2,2',2'',2'''-(4,7-diphenyl-1,10-phenanthroline-2,9-diyl) bis (methylene) bis (azanetriyl) tetra acetic acid (BC-EDTA) was synthesized and characterized. It shows an emission spectrum peak at 610 nm when it is excited at 360 nm, with a large Stock shift (250 nm). It is covalently coated on the surface of a bare silica nanosphere containi free amino groups, using 1-ethyl-...
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 1999
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.15.121